Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Identification, cloning, and recombinant expression of procalin, a major triatomine allergen.

doi: 10.4049/jimmunol.167.5.2694

Figure Lengend Snippet: FIGURE 5. Immunolocalization of 19-kDa salivary allergen of T. pro- tracta. Salivary glands or whole bugs were fixed, embedded, and sectioned as described in Materials and Methods. A, Control Ab; B, anti-procalin rabbit antisera (1:1000) as primary Ab. Alkaline phosphatase-linked sec- ondary Ab (goat anti-rabbit) reaction localizes allergen to salivary gland epithelial cells and contents of salivary gland lumen (original magnifica- tion, 200).

Article Snippet: Sections (2.5 m) were cut and incubated overnight at 4°C with a 1/1000 dilution of hyperimmune rabbit antiserum reactive with the recombinant protein, which were washed and incubated with a 1/100 dilution of alkaline phosphatase-labeled goat anti-rabbit Ab by using the Vectastain ABC (avidin-biotin complex)-alkaline phosphatase kit (Vector Laboratories, Burlingame CA) (17, 18).

Techniques: Control